Journal: Toxicology Reports

Article Title: A toxicity profile of the Pheroid® technology in rodents

doi: 10.1016/j.toxrep.2019.08.012

Figure Lengend Snippet: Light microscopy images of organs from the sub-chronic treatment group (pro-Pheroid®) with a) liver b) lung c) kidney and d) heart. All tissues were stained with Haematoxylin and Eosin.

Article Snippet: Morphological conformation was determined by confocal laser scanning electron microscopy (CLSM, Nikon D-eclipse C1 confocal scanning microscope, United States) as per the method outlined by Slabbert et al, .

Techniques: Light Microscopy, Staining

Journal: Biomicrofluidics

Article Title: Microfluidic model of monocyte extravasation reveals the role of hemodynamics and subendothelial matrix mechanics in regulating endothelial integrity

doi: 10.1063/5.0061997

Figure Lengend Snippet: Microfluidic platform for investigating monocyte extravasation. (a) Photograph of device bonded to (24 × 40 mm 2 coverslip). (b) Graphical representation of the device (not to scale) that consists of a central chamber with two ports (dark green) through which the collagen gel (light green) is introduced and polymerized. A channel formed by an acupuncture needle connects two reservoirs filled with the medium (bright pink) to the collagen gel region. HUVECs line the channel in the collagen gel (pink), and hydrostatic pressure gradients induced by the rocker induce flow through the channel. Monocytes (blue) are flowed through the vessel, and extravasation from the lumen into the collagen hydrogel is investigated using light microscopy.

Article Snippet: Fresh EGM-2 was introduced into devices, and devices were moved to a laboratory rocker within a humidified incubator to wash devices for 24 h. The next day, a HUVEC suspension with a final concentration of 2 × 10 6 cells/ml in EGM-2 was introduced into the device reservoirs, and cell adherence to the central channel in the collagen gel was observed by phase contrast microscopy (Nikon D-Eclipse C1 Confocal Microscope, 10× lens, Nikon Instruments, Tokyo, Japan).

Techniques: Light Microscopy

Journal: Biomicrofluidics

Article Title: Microfluidic model of monocyte extravasation reveals the role of hemodynamics and subendothelial matrix mechanics in regulating endothelial integrity

doi: 10.1063/5.0061997

Figure Lengend Snippet: 3D structure and hydraulic permeability of 2.5 and 6 mg/ml collagen gels. (a) Scanning electron microscope (SEM) images of 2.5 and 6 mg/ml gels at resolutions of 10, 20, 50, and 100 K. (b) Diagram of two-channel device used to measure hydraulic permeability. Reservoirs are connected to the media ports to allow application of a defined pressure gradient across the collagen hydrogel. (c) The experimental setup of fluorescence recovery after photobleaching (FRAP) method used to measure the hydraulic permeability. A known pressure gradient is applied to hydrogels immersed in the dextran-containing medium, and the velocity magnitude of a photobleached circle is measured with timelapse fluorescence microscopy. (d) Sample FRAP data demonstrating spot displacement in response to an applied hydrostatic pressure gradient. (e) Measured velocity of fluid flow between two channels. (f) Hydraulic permeability of collagen hydrogels as a function of applied pressure gradient calculated from Darcy's law. For all plots, each data point represents data from an individual device, and solid and dashed red lines represent the median and mean values, respectively. ANOVA tests were performed to determine statistical significance. ***p < 0.001.

Article Snippet: Fresh EGM-2 was introduced into devices, and devices were moved to a laboratory rocker within a humidified incubator to wash devices for 24 h. The next day, a HUVEC suspension with a final concentration of 2 × 10 6 cells/ml in EGM-2 was introduced into the device reservoirs, and cell adherence to the central channel in the collagen gel was observed by phase contrast microscopy (Nikon D-Eclipse C1 Confocal Microscope, 10× lens, Nikon Instruments, Tokyo, Japan).

Techniques: Permeability, Microscopy, Fluorescence